The use of Aloe barbadensis M. (Aloe vera) Extract as Potential Stains in Gram’s Staining Technique

Aloe barbadensis miller (Aloe vera) aside being a potent antioxidant with effects attributed to the phytoconstituents (anthraquinone), can also serve as natural dyes. This study explored staining potentials of A. extract on microbial specimens in modified Gram’s technique. The plant was washed; shrubby edges leaves were scraped off and each leaf cut open expel gel content. dried grinded material weighing 450g treated two different extraction methods (150g for heated alcoholic aloe (HAAE) 150g unheated extracts I & II (UAAE-1 UAAE-II). Preliminary phytochemical screening using bontragers test conducted. Solutions from without mordant applied bacterial smears. Anthraquinone-rich under optimal conditions 10.0, 4.84 18.8 respectively. Comparing methods, HAAE UAAE1 UAAEII are mutually convenient easy make; but former more cost-effective regards power instrumentation while latter cost-efficient solvent. pH various prepared solutions acidic contained bioactive agent that imparts pigment biological specimens. In comparing mordanted non-mordanted solutions, had no additional efficiency dye extract. We concluded visibly stained Gram positive negative cells structural differentiations. recommend reductions duration samples ascertain reasons variation uptake.